Figure 3.

Circ-PITX1 silencing restrained the glycolysis and glutamine metabolism of NSCLC cells in vitro. HCC827 and H1650 cells were transfected with si-NC or si-circ-PITX1. (A–C) The glucose consumption, lactate production and ECAR were measured to assess cell glycolysis. (D–F) Glutamine consumption, α-KG production and ATP production were determined using corresponding Assay Kits, respectively. (G and H) WB analysis was performed to assess the protein expression of HK-2 and GLS1. *P < 0.05, **P < 0.01.