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. 2021 Mar 9;14:1807–1819. doi: 10.2147/OTT.S286820

Figure 4.

Figure 4

Circ-PITX1 could sponge miR-1248. (A) After transfected with si-NC or si-circ-PITX1 into HCC827 and H1650 cells, the expression of candidate miRNAs (miR-185-3p, miR-326-3p, miR-1248 and miR-512-3p) was measured by qRT-PCR. (B) The sequences of binding sites and mutated sites between miR-1248 and circ-PITX1 were presented. Dual-luciferase reporter assay (C and D) and RIP assay (E and F) were used to verify the interaction between miR-1248 and circ-PITX1. (G) QRT-PCR was performed to test the expression of miR-1248 in 41 paired NSCLC tumor tissues (Tumor) and adjacent normal tissues (Normal). (H) The expression of miR-1248 in BEAS-2B cells and NSCLC cell lines (HCC827 and H1650) was measured by qRT-PCR. (I) HCC827 and H1650 cells were transfected with vector, circ-PITX1, si-NC or si-circ-PITX1. The expression of miR-1248 was detected by qRT-PCR. *P < 0.05, **P < 0.01, ***P < 0.001.