Figure 5.

Circ-PITX1 regulated NSCLC progression by sponging miR-1248. HCC827 and H1650 cells were transfected with si-NC, si-circ-PITX1, si-circ-PITX1+ anti-miR-NC or si-circ-PITX1 + anti-miR-1248. (A) The expression of miR-1248 was measured by qRT-PCR. The viability, colony number, apoptosis, migration and invasion of cells were determined using CCK8 assay (B), colony formation assay (C), flow cytometry (D), wound healing assay (E) and transwell assay (F). (G) Flow cytometry also was used to detect cell cycle process. (H–J) Cell glycolysis was assessed by detecting the glucose consumption, lactate production and ECAR. (K–M) Glutamine consumption, α-KG production and ATP production were evaluated by the corresponding Assay Kits, respectively. (N and O) The protein expression of HK-2 and GLS1 was assessed using WB analysis. *P < 0.05, **P < 0.01.