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. 2021 Mar 9;14:1807–1819. doi: 10.2147/OTT.S286820

Figure 6.

Figure 6

CCND2 was targeted by miR-1248. (A) The sequences of binding sites and mutated sites between CCND2 3ʹUTR and miR-1248 were exhibited. The interaction between CCND2 and miR-1248 was confirmed using dual-luciferase reporter assay (B) and RIP assay (C). (D–G) The mRNA and protein expression levels of CCND2 in 41 paired NSCLC tumor tissues (Tumor) and adjacent normal tissues (Normal), as well as in BEAS-2B cells and NSCLC cell lines (HCC827 and H1650) were measured by qRT-PCR and WB analysis. (H) The transfection efficiencies of miR-1248 mimic and inhibitor were evaluated by measuring miR-1248 expression in HCC827 and H1650 cells using qRT-PCR. (I) HCC827 and H1650 cells were transfected with miR-NC, miR-1248, anti-miR-NC or anti-miR-1248. The protein expression of CCND2 was examined by WB analysis. *P < 0.05, **P < 0.01, ***P < 0.001.