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. 2021 Mar 9;14:1807–1819. doi: 10.2147/OTT.S286820

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© 2021 Yue et al.

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MiR-1248 and CCND2 overexpression regulated NSCLC progression. HCC827 and H1650 cells were transfected with miR-NC, miR-1248, miR-1248 + pcDNA or miR-1248 + CCND2. (A and B) The mRNA and protein expression of CCND2 was tested using qRT-PCR and WB analysis. CCK8 assay (C), colony formation assay (D), flow cytometry (E), wound healing assay (F) and transwell assay (G) were performed to detect the viability, colony number, apoptosis, migration and invasion of cells. (H and I) Flow cytometry was employed to measure the cell cycle process. (J–L) Cell glycolysis was determined using analyzing the glucose consumption, lactate production and ECAR of cells. (M–O) The corresponding Assay Kits were used to determine the glutamine consumption, α-KG production and ATP production, respectively. (P and Q) The protein expression of HK-2 and GLS1 was evaluated by WB analysis. *P < 0.05, **P < 0.01.