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. 2021 Mar 9;15:1075–1089. doi: 10.2147/DDDT.S275228

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© 2021 Bi et al.

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The effect of Polygonum cuspidatum extraction on ASM cells proliferation and apoptosis. (A) ASM cells were treated with PDGF (10 ng/mL) and Polygonum cuspidatum extraction (0.25 mg/mL, 0.5 mg/mL, 1 mg/mL and 2 mg/mL) at different concentrations alone or in combination for 24h. Cell proliferation was detected by CCK-8. The data were expressed as mean ± SD (n=6) of each group. (B, C) ASM cells were treated with Polygonum cuspidatum extraction (0.25 mg/mL, 0.5 mg/mL, 1 mg/mL and 2 mg/mL) for 24h. The apoptosis of ASM cells were detected by Annexin-V staining. The data were expressed as mean ± SD (n=3) of each group. ^ns P>0.05 vs PDGF group or control group and ** P<0.01 vs PDGF group or control group, #P<0.01vs control group.