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. 2021 Mar 9;16:1993–2011. doi: 10.2147/IJN.S277014

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© 2021 Piktel et al.

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Western blot evaluation of apoptotic (A and B) and autophagic (C and D) proteins expression in human ovarian adenocarcinoma SKOV-3 cells upon treatment with peanut-shaped gold nanoparticles (AuP NPs) of 5 ng/mL for 24 hours. In this experiment, cells were pre-treated for 2 hours with signaling pathways inhibitors: apoptosis inhibitor (Z-VAD-FMK, 20 uµM), or autophagy inhibitor (3-MA, 5 mM). Results are presented as mean±SD from three independent experiments. *Statistical significance (P<0.05) when compared to samples treated with AuP NPs without pre-incubation with inhibitor.