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. 2020 Oct 15;19(3):548–562. doi: 10.1111/pbi.13484

Figure 2.

Figure 2

GhARF16‐1 is involved in regulating leaf shape in cotton. (a)–(c) Top, from left to right, 48‐d‐old phenotypes of wild‐type Gossypium hirsutum (a), GhARF16‐1 RNAi (b), and pARF16‐1::GhmARF16‐1 transgenic G. hirsutum plants (c). Bars = 4 cm. Bottom, from left to right, scanning electron micrographs of the leaf epidermal cells at the base of the abaxial side of mature leaves of wild‐type, GhARF16‐1 RNAi, and pARF16‐1::GhmARF16‐1 transgenic lines. Bars = 50 µm. (d) Close‐up view of the leaves of 48‐d‐old wild‐type, GhARF16‐1 RNAi, and pARF16‐1::GhmARF16‐1 transgenic plants. (e)–(g) Quantitative comparisons of the leaf shapes of wild‐type, GhARF16‐1 RNAi plants, and pARF16‐1::GhmARF16‐1 transgenic lines based on the leaf dissection index (perimeter2/4π × leaf area) (e), the number of teeth/leaf perimeter (f), and the tooth area/leaf area (g). (h) Statistical analysis of number of teeth per leaf of wild‐type, GhARF16‐1 RNAi, and pARF16‐1::GhmARF16‐1 transgenic plants. A total of 10 leaves from each line were used for each measurement. Data are presented as mean ± SE. Statistical significance was determined using one‐way analysis of variance combined with Tukey's test. **P < 0.01; ***P < 0.001.