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. 2020 Oct 15;19(3):548–562. doi: 10.1111/pbi.13484

Figure 6.

Figure 6

Ectopic expression of GhKNOX2‐1 leads to lobed leaves in Arabidopsis thaliana. (a)–(d) Top, from left to right, 21‐d‐old phenotypes of wild‐type A. thaliana (a), knat2 mutant (b) and 35S::GhKNOX2‐1 transgenic Arabidopsis (c). Bars = 4 mm. Bottom, from left to right, scanning electron micrographs of the leaf epidermal cells at the base of the abaxial side of mature leaves of wild‐type, knat2 mutant and 35S::GhKNOX2‐1 transgenic Arabidopsis. Bars = 50 µm. (d) Close‐up images of the leaves of 21‐d‐old wild‐type (top), knat2 mutant (middle) and 35S::GhKNOX2‐1 transgenic Arabidopsis (bottom). (e)–(g) Quantitative comparisons of the leaf shapes of wild‐type, knat2 mutant and 35S::GhKNOX2‐1 transgenic lines based on the leaf dissection index (perimeter2/4π × leaf area) (e), number of teeth/leaf perimeter (f) and tooth area/leaf area (g). (h) Statistical analysis of the number of teeth per leaf of wild‐type, knat2 mutant and 35S::GhKNOX2‐1 transgenic Arabidopsis. A total of 10 leaves of each phenotype were used for each measurement. Data are presented as the mean ± SE. Statistical significance was determined using one‐way analysis of variance combined with Tukey's test. *P < 0.05; ***P < 0.001. WT, wild type.