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. 2021 Mar 14;1865(6):129893. doi: 10.1016/j.bbagen.2021.129893

Fig. 2.

Fig. 2

Coomassie Blue stained SDS-PAGE and western blot probed with anti-SARS-CoV-2 Spike rabbit antibody. (A) SDS-PAGE gel of 10 μL fermentation supernatant for tagged-free RBD219-WT, RBD219-N1, and RBD219-N1C1; (B) Coomassie Blue stained SDS-PAGE gel of 3 μg purified RBDs or western blot of 1.5 μg of the purified RBDs under non-reduced and reduced conditions. (C) SDS-PAGE of 3 μg PNGase-F treated purified RBDs; please note that the 37 kDa band observed on the PNGase-F treated gel is the N-glycosidase PNGase-F enzyme.