Table 1.

Assessment of the fermentation supernatants for clones RBD219-219, RBD219-N1, and RBD219-N1C1.

Molecule	Fermentation yield⁎ (mg RBD/L of FS)	Level of Impurity on SDS-PAGE	Hyperglycosylation on WB	Dimer formation on WB
RBD219-WT	142 ± 8	High	Yes	Yes
RBD219-N1	50 ± 13	Mid	No	Yes
RBD219-N1C1	280 ± 70	Low	No	Yes

^⁎Fermentation yield was measured by probing the RBD with the same specific antibody followed by densitometry (Supplementary Fig. S1). FS: fermentation supernatant; WB: western blot.