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. Author manuscript; available in PMC: 2021 Sep 15.
Published in final edited form as: Clin Cancer Res. 2020 Dec 3;27(6):1766–1777. doi: 10.1158/1078-0432.CCR-20-1499

Figure 2. Radiation-induced upregulation of betacellulin (BTC) and bone morphogenic protein 4 (BMP-4) contributes to MSC migration to DIPG tumors.

Figure 2.

A) Protein array of SF8628 DIPG cells isolated from the brainstem of control and irradiated mice (n=3 per control and irradiated groups). Heat map values for each protein represent an average of two independent reads of the array. Representative flow cytometry histogram of MSCs for expression of B) EGFR-epidermal growth factor (n=3) and C) BMPR2-bone morphogenic protein receptor 2 (n=3), receptors for BTC and BMP-4 ligands, respectively. D) Quantitative PCR assessment of the mRNA levels of expression of BTC and BMP-4 after siRNA-induced gene silencing (n=3, One-way ANOVA with Tukey’s post hoc test. Levels of significance are: ** p <0.01, *** p <0.001 and **** p<0.0001). E) and F) Migration of MSCs toward irradiated SF8628 and DIPG007 cells, respectively, after silencing of both BTC and BMP-4 siRNA in vitro. (One-way ANOVA with Tukey’s post hoc test, n≤4. Level of significance: * p <0.05, ** p <0.01).