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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Gastroenterology. 2020 Nov 21;160(4):1301–1314.e8. doi: 10.1053/j.gastro.2020.11.034

Figure 6. F. nucleatum strains aggregate with C. difficile R20291 on HT29-MTX cells and human colonoids.

Figure 6.

A. Representative images of mucin producing HT29-MTX monolayers incubated with CFDA-SE fluorescently tagged C. difficile R20291 (green) and CMRA fluorescently-tagged F. nucleatum subspecies nucleatum, nucleatum Δfap2, nucleatum ΔradD, polymorphum or animalis (red) after 1 hr (scale bar =50 μm)(n=3 replicates, repeated 3 independent times). B. Representative images of human colonoids (organoids) incubated with C. difficile R20291 (green) and F. nucleatum subspecies nucleatum after 1 hr (scale bar =50 μm) (n=3 replicates, repeated 2 independent times). Higher magnification inset below highlights co-localization. C. Representative image of C. difficile and Fusobacterium FISH staining in surgical resections from patients with recurrent CDI (n=4 patients). Positive staining for C. difficile (green) and Fusobacterium (red) were observed at 40x (left) (scale bar = 50 μm) and 400x (inset right) (scale bar = 100 μm)