Fig. 6. BCR signaling activated RNA pol II and increased Mcl-1 levels; this was blocked by TG02.

A CLL cells were incubated with TG02 or Lcki for 1 h before stimulating with anti-IgM for 1 h and 24 h. The phosphorylation RNA pol II and the levels of anti-apoptotic proteins were evaluated by immunoblotting. The mean CLL cell viability of three individual experiments assessed at 24 h is shown below the image. B Inhibiting Lck had minimal toxicity on the CLL cells. CLL cells were incubated with Lcki for 1 h before stimulating with anti-IgM for 24 h. The percentage of surviving cells with or without Lcki was compared in groups without (white bars) and with (gray bars) anti-IgM stimulation (mean ± SEM, n = 8).