Skip to main content
. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Dev Biol. 2021 Jan 11;472:30–37. doi: 10.1016/j.ydbio.2020.12.019

Figure 4. FGF signaling is required for activation of AKT signaling and cardiomyocyte survival.

Figure 4

(A) Schematic presentation of experimental design. The red bars represent dnfgfr1 induction by pulses of heat shock.

(B-G) Confocal images of the pAKT and Mef2 signals in the ventricle without amputation (B, C) and after amputation (D-G) in WT (B, D, F) and hsp: dnfgfr1 fish (C, E, G).

(H-M) Confocal images of the Mef2 and TUNEL signals in the ventricle without amputation (H, I) and after amputation (J-M) in WT (H, J, L) and hsp: dnfgfr1 fish (I, K, M).

(N) Graphic presentation of the percentage of the pAKT-positive CMs among CMs in the apex at indicated time points.

(O) Graphic presentation of the percentage of the TUNEL-positive CMs among CMs in the apex at indicated time points.

Dotted lines in D-G and J-M indicate amputation planes. The areas in yellow squares in B-M are shown on the right side. Arrowheads point to double positive cells. Scale bar, 100 μm. * p<0.05, ** p<0.01 by one-way ANOVA with Tukey HSD. n.s., not significant (p≥0.05). n=3 for no amputation, 1 dpa and 3 dpa in both WT and hsp: dnfgfr1 fish in N and O. Each circle in graphs represents an average value for each animal.