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. 2021 Feb 25;22(5):2274. doi: 10.3390/ijms22052274

Figure 6.

Figure 6

(A,B) SH-SY5y cells (1 × 105/well, 6-well plate) were exposed to the indicated concentration (0 to 2 mM) of METH for 6 h or 2 mM of METH for the indicated time (0 to 6 h). After incubation, cells were collected for real-time quantitative (PCR analysis. The mRNA levels of Beclin1 (A) and LC3 (B) were detected and normalized with the level of GAPDH. (C) SH-SY5y cells (1 × 105/well, 6-well plate) were pre-treated with the indicated concentration of aromadendrin (0 to 40 μM) for 1 h and exposed to 2 mM METH for 6 h. After incubation, cells were harvested and mRNA levels of Beclin1 and LC3 were determined by real-time quantitative PCR analysis. (D) SH-SY5y cells (1 × 105/well, 6-well plate) were pre-treated with the indicated concentration (0 to 40 μM) of aromadendrin for 1 h and exposed to 2 mM METH for 24 h. Cells were collected for Western blot analysis and indicated proteins were detected. The levels of indicated proteins were normalized with the level of β-actin. The mean value of three experiments ± SEM is presented. * p < 0.05 between mock-treated cells (A,B) or METH-treated cells (C,D).