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. 2021 Feb 25;22(5):2274. doi: 10.3390/ijms22052274

Figure 7.

Figure 7

(A) SH-SY5y cells (5 × 103/well, 96-well plate) stained with 1 × AnnexinV staining reagent were pre-treated with the indicated concentration (0 to 40 μM) of aromadendrin for 1 h and exposed to 2mM METH for 24 h. AnnexinV was detected by IncuCyte® imaging system, and the intensity of AnnexinV was calculated. (B) SH-SY5y cells were pre-treated with the indicated concentration (0 to 40 μM) of aromadendrin for 1 h and exposed to 2 mM METH for 24 h. After collection, AnnexinV/PI apoptosis assay was performed by flow cytometry, and AnnexinV/PI-positive cells were presented in a bar graph. White bar indicates 0.2 mm. The mean value of three experiments ± SEM is presented. * p < 0.05 between METH-treated cells.