Table 7.
Effects of 24 h, 48 h, and 6 days exposures to TCS on TNFα secretion from PBMCs.
| 24 h | TNFα secretion in pg/mL (mean±S.D.) | |||
|---|---|---|---|---|
| [TCS]μM | F214 | F224 | F509 | F510 |
| 0 | 669±29 | 290±7 | 670±108 | 595±10 |
| 0.05 | 1082±234 | 324±60 | 994±116+ | 519±47 |
| 0.1 | 917±80+ | 231±88 | 663±48 | 560±10* |
| 0.25 | 1327±425 | 351±27 | 885±66 | 621±4+ |
| 0.5 | 613±47 | 336±25 | 1041±59+ | 620±3+ |
| 1 | 1040±658 | 351±23+ | 1013±22+ | 622±8+ |
| 2.5 | 773±122 | 368±55 | 1387±81+ | 726±10+ |
| 5 | 800±24+ | 980±34+ | 4322±198+ | 941±13+ |
| 48 h | TNFα secretion in pg/mL (mean±S.D.) | |||
| [TCS]μM | F214 | F224 | F509 | F510 |
| 0 | 40±7 | 153±7 | 218±2 | 243±18 |
| 0.05 | 593±69+ | 169±3+ | 450±46+ | 209±23 |
| 0.1 | 698±82+ | 115±12* | 287±8+ | 220±22 |
| 0.25 | 529±24+ | 140±4 | 419±24+ | 220±9 |
| 0.5 | 610±110+ | 157±22 | 281±3+ | 235±11 |
| 1 | 331±31+ | 138±5 | 345±15+ | 227±11 |
| 2.5 | 624±34+ | 173±10 | 243±12 | 238±30 |
| 5 | 681±41+ | 929±59+ | 890±8+ | 341±16+ |
| 6 d | TNFα secretion in pg/mL (mean±S.D.) | |||
| [TCS]μM | F214 | F224 | F509 | F510 |
| 0 | 27±3 | 52±7 | 419±13 | 231±21 |
| 0.05 | 12±2* | 18±3* | 193±12* | 269±21 |
| 0.1 | 17±4* | 29±9* | 154±9* | 357±50+ |
| 0.25 | 18±1* | 32±2* | 305±23* | 248±33 |
| 0.5 | 15±1* | 26±3* | 344±8* | 510±29+ |
| 1 | 11±1* | 17±1* | 519±26+ | 522±40+ |
| 2.5 | 25±1 | 48±3 | 607±40+ | 514±6+ |
| 5 | 294±8+ | 651±18+ | 333±10* | 799±35+ |
Values are mean±S.D. of triplicate determinations.
+
indicates a significant increase and
*
indicates a significant decrease in secretion compared to control cells, p<0.05