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. 2021 Feb 25;13(5):958. doi: 10.3390/cancers13050958

Figure 2.

Figure 2

Idasanutlin sensitizes macrophages to apoptosis by the Fas-stimulating antibody. (A,B) Western analysis and quantification of caspase-7 cleavage (A) and caspase-3/7 activity (B) in macrophages pre-treated with 250 nM RG7388 for 24 h followed by treatments with 1 µg/mL Fas antibody or 10 ng/mL TNFα in the presence of 10 µg/mL cycloheximide (CHX) for 6 h (n = 4). (C) Western blot analysis and quantification of caspase-7 cleavage in macrophages pre-treated with 250 nM RG7388 for 24 h followed by treatments with doxorubicin for 24 h (n = 5).