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. Author manuscript; available in PMC: 2021 Apr 19.
Published in final edited form as: Nat Biotechnol. 2020 Oct 19;39(3):357–367. doi: 10.1038/s41587-020-0707-9

Extended Figure 3. Comparison on the cytotoxicity, OCR inhibition and mitochondria membrane potential damaging effect between CDN and established complex I inhibitors.

Extended Figure 3.

MDA-MB-231 or MDA-MB-468 cells were incubated with 1 μM of CDN, IACS-010759 or BAY 87–2243. (a) Cell viability after 24 h of treatment was measured by MTS assay (mean ± s.e.m., n=3 biologically independent samples, P values from unpaired t test, two-tailed). (b) OCR and (c) ECAR were determined via Seahorse assay at 1 h after incubation. The results were normalized by cell number (mean ± s.e.m., n=6 biologically independent samples, P values from unpaired t test, two-tailed)). (d) Representative confocal microscopy images of cells stained with MitoTracker Green (MT-G, green) and DAPI (blue) after 24 h treatment. (scale bar: 50 μm). Three experiments were repeated independently with similar results.