Figure 4. Nasotemporal and optic fissure defects of Pax2^GFP/GFP mutants.

All panels show sagittal view of eye. (A,A',F,F') Vsx2-Mitf colabeling of E11.5 sections. In the ventronasal optic, Mitf domain is expanded at the expense of Vsx2/Chx10. (B,F) At E11.5, Foxg1 immunostaining also highlights proportionally smaller nasal side of Pax2^GFP/GFP optic cup. (C,G) In E13.5 Pax2 mutants, optic cup size and the Foxg1 domain better resemble controls, but nasal tip is abnormally positioned, emphasizing failed fissure closure (D,H) E13.5 Anti-laminin labeling of the basement membrane also highlights failed fissure closure of Pax2^GFP/GFP eyes (arrow in H). n = 3 biologic replicates/genotype Scalebar = 50μm