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. 2021 Feb 24;22(5):2251. doi: 10.3390/ijms22052251

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Characterization of nanoparticles by using the chorioallantoic membrane (CAM) assay. (A) Tumor formation on the CAM by transplanting cancer cells; (B) A CAM-based assay to examine tumor accumulation of nanoparticles. The tumor is formed using green fluorescence protein (GFP) expressing cells; (C) Detection of ¹⁰BPA-BPMO in tumor and organs.