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. 2021 Feb 26;22(5):2340. doi: 10.3390/ijms22052340

Figure 1.

Figure 1

Senescence marker protein 30 (SMP30) levels in glandular epithelial cells correlate with malignancy of mammary gland tumors in dogs and cats. (A) Representative images of hematoxylin and eosin (H&E) staining and immunohistochemistry stained with anti-SMP30 normal tissue, adenoma, carcinoma (grade 1), and mammary carcinoma (grade 2). Significantly higher SMP30 expression was observed with an increase in malignancy of mammary gland tumors. Scale bars = 100 μm. Inset, scale bars = 50 μm. (B) Immunohistochemistry intensity scoring for SMP30 expression examined in canine mammary gland tumors. SMP30 expression significantly increased in carcinoma whereas adenoma showed a low level of SMP30 (** p < 0.01). (C) Representative images of H&E staining and immunohistochemistry of mammary specimens from felines with mammary carcinoma (grade 1) and mammary carcinoma (grade 2). A higher SMP30 expression level was observed in glandular epithelial cells of mammary carcinoma (grade 2) than that of mammary carcinoma (grade 1). Scale bars = 100 μm. Inset, scale bars = 50 μm. (D) Immunohistochemistry intensity scoring for SMP30 expression in feline mammary gland tumors. SMP30 expression significantly increased in grade 2 carcinoma compared to grade 1 carcinoma, indicating that SMP30 expression corresponds to the malignancy of neoplastic epithelial cells (** p < 0.01).