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Skeletal muscle cell differentiation activity of compound 1 on C2C12 differentiated cells. The levels of p-PI3K p85, p-Akt, p-FoxO1, p-mTOR, p-p70S6K, and MyoD proteins were determined using a Western blot analysis. Every level of proteins versus β-Actin were measured by densitometry. C, control. Experiments were performed in triplicate and the data are expressed as mean ± SE; ** p < 0.01 as compared to the control (untreated cells).
