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Skeletal muscle cell differentiation activity of compound 1 on C2C12 differentiated cells. The levels of p-Smad2/3 and Smad4 proteins were determined using a Western blot analysis. Every level of proteins versus β-Actin were measured by densitometry. C, control. Experiments were performed in triplicate and the data were expressed as mean ± SE; ** p < 0.01 as compared to the control (untreated cells).
