Figure 6.

The chondrogenic differentiation of ADSCs induced by KGN/rGO@Ge complex media. (A) The evaluation of different K/G ratio media on the ADSCs chondrogenic differentiation. The Ab and Tb stainings were used to test the effects of different K/Gs (1 μM KGN corresponding to 0, 10, 10², 10³ and 10⁴ μg/mL rGO@Ge) medium, bar: 500 μm. (B) The quantitative analysis of the alcian blue staining to evaluate the GAG content. * p < 0.05 indicates a significant difference compared to the K/G0 group. Immunofluorescence staining of the chondrogenic differentiation marker Sox-9 (C) and Col II (D) to clarify the ADSCs chondrogenesis, Bar: 200 μm. (E) The PCR analysis on the expression of the chondrogenic differentiation markers in ADSCs cultured with the different K/G media. * p < 0.05 indicates a significant difference compared to the K/G0 group.