Figure 1.

Tissue preservation ex vivo. (A) Tissue culture maintains its architecture and cellular composition throughout the cultivation period of 14 days. Tissue was fixated at the indicated days, embedded in paraffin and stained with H&E. Here, tissue was cut horizontally, 5 µm thick. Bar = 100 µM. The tumor fraction was stable up to day 14. Each dot represents one specimen (n = 3), d = day. Day 0 was set to 100%. Error bars show SEM (B) Tissues were stained with antibodies against CD3, CD8 and CD68 on day 2, 4 and 7 and were compared to day 0. Bar = 100 μm. (n = 3). One-way ANOVA (p < 0.05) showed no significant alterations.