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Schematic representation of (a) DPN (DOX-loaded PLGA nanoparticle), (b) iDPN (PEI was added in W1 during the incorporation process), i’DPN (PEI was added in O during the incorporation process), (c) IDPN (PEI-DPN), and AIDPN (PAA-PEI-DPN). Abbreviations: DCM, dichloromethane; DOX, Doxorubicin hydrochloride; W1, 0.5% DOX aqueous solution; W2, outer water, which was water or PB (phosphate buffer; pH 8); PEI, polyethylenimine; PAA, poly(acrylic acid).
