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. 2021 Feb 24;26(5):1211. doi: 10.3390/molecules26051211

Figure 3.

Figure 3

Concentration-dependent inhibition responses of mutants to chanoclavine. (A–D). Y91A, F130A, N138A, and F130A+N138A mutant receptors are rectified at a −80 mV holding potential. The 5-HT3A mutant receptors expressed in Xenopus oocytes elicit reversible currents using an electrode voltage clamp. All oocytes were exposed to 10, 30, 100, and 300 µM chanoclavine coapplied with 100 µM 5-HT after treatment with 5-HT alone. (E). The inhibition percentage curve according to the EC concentration of the 5-HT3A mutant receptors. Each value represents the mean ± SEM. Additional half-maximal inhibitory response concentrations (IC50), Imax, and Hill’s coefficient values of the other mutants are listed in Table 1 (n = 6–8 from four different frogs).