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. 2021 Feb 24;22(5):2260. doi: 10.3390/ijms22052260

Figure 4.

Figure 4

Validation of the relative expression level of partial known and novel miRNAs by sRNA sequencing and qPCR in PA64S(S) and PA64S(F). Dark blue lines indicate relative changes in the expression levels of three differentially expressed novel miRNAs, as determined by RNA-Seq. Blue bars indicate relative changes in the expression levels of the novel miRNAs, as determined by qPCR. Red lines indicate relative changes in the expression levels of three differentially expressed known miRNAs, as determined by RNA-Seq. Pink bars indicate relative changes in the expression levels of the known miRNAs, as determined by qPCR. The average values of three biological repeats are shown. Error bars represent standard deviations. Asterisks indicate significant differences revealed by Student’s t-test at p < 0.05 (*).