Table 5.

Sequences of primers used for the RT-PCR analysis.

Gene	Primer Sequence Forward (5ʹ→3ʹ)	Primer Sequence Reverse (5ʹ→3ʹ)	bp
TNF-α	TGC TTG TTC CTC AGC CTC TT	TGA GGT ACA GGC CCT CTG AT	218
IL-1β	CGA TGC ACC TGT ACG ATC AC	TCT TTC AAC ACG CAG GAC AG	226
HIF-1α	TTG CCT TTC CTT CTC TTC TCC	CAA TCC AAG GTT GCC AAG TT	164
VEGF	CCT TGC TGC TCT ACC TCC AC	ATC CAC CCC AAA ACT TTT CC	236
TGF-β	ACA TTG ACT TCC GCA AGG AC	CCG GGT TAT GCT GGT TGT A	150
PIGF	GTT CAG CCC ATC CTG TGT CT	AGC AGG GAA ACA GTT GGC TA	244
IL-6	TGC GTC CGT AGT TTC CTT CT	GGA ATC TTC TCC TGG GG GTA	211
GAPDH	GAT GGT GAA GGT CGG TGT G	ATG AAG GGG TCG TTG ATG G	108

GAPDH—glyceraldehyde 3-phosphate dehydrogenase.