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. 2021 Feb 25;22(5):2309. doi: 10.3390/ijms22052309

Figure 1.

Figure 1

Patients with acute tubular necrosis (ATN) and acute renal injury display increased expression of renal tubular transient receptor potential ankyrin 1 (TRPA1) and 8-hydroxy-2′-deoxyguanosine (8-OHdG), a biomarker of oxidative stress. (A) Representative images showing immunostaining with an antibody against TRPA1 or 8-OHdG. Renal sections were obtained from normal control subjects and patients with ATN. The specificity of the immunostaining was confirmed by using an IgG-negative control. The magnification of each panel was 400×. (B) Quantification of the expression levels of renal tubular TRPA1 and 8-OHdG in the renal sections with immunostaining. Data in each group are the mean ± SEM from 10 patients. ** p < 0.01 versus the control group. (C,D) Pearson correlation analyses of expression of renal tubular TRPA1 or 8-OHdG with tubular injury score in patients with ATN (n = 10); r represents the correlation coefficient. Tubular injury score was calculated according to the percentage of injured area of tubular cross sections. The scoring system is described in the Methods section.