Figure 6.

TRPA1 mediated the induction of IL-8 by H/R in HK-2 cells. (A) HK-2 cells were incubated under normoxia conditions as control or exposed to 2.5% O₂ for 6 h, followed by 4 h of reoxygenation. In 6 study groups, cells were pretreated with 3, 6, or 9 µM HC-030031 (HC3, HC6 or HC 9; a TRPA1 antagonist). (B) HK-2 cells were incubated under normoxia conditions with or without the application of TRPA1 siRNA (siTRPA1) at two concentrations (25 and 50 nM) 48 h prior to the measurement of TRPA1 expression. Protein expression was analyzed by Western blot. (C) HK-2 cells were incubated under normoxia conditions as control or exposed to 2.5% O₂ for 6 h, followed by 4 h of reoxygenation. In the 4 study groups, cells were pretreated with 50 nM of siTRPA1 or scramble siRNA. Protein expression was analyzed by ELISA. Data in each group are mean ± SEM from 6 independent experiments. * p < 0.05 versus the control group; ** p < 0.01 versus the control group; ^# p < 0.05 versus the H/R group without pretreatment of siRNA; ^## p < 0.01 versus the H/R group without pretreatment of HC.