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Detection of apoptosis and localization of EFCC4-positive granular amoebocytes in the 4-week anterior (a) and posterior (b) blastema. The TUNEL positive cells were overwhelmingly increased after 4 weeks and granular amoebocytes (red) were also present in the close vicinity of apoptotic cells (green). CC-coelomic cavity. Arrows point to TUNEL positive cells. Representative images were presented from five independent experiments. Scale bars: 100 μm.
