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. 2021 Feb 26;13(5):980. doi: 10.3390/cancers13050980

Figure 6.

Figure 6

Figure 6

EFNB2 and PBX1 were commonly downregulated in human gynaecologic cancer cells with sfTSLP overexpression. (a) The volcano plot showed the upregulated (red dots) and downregulated (green dots) differentially expressed genes (DEGs) of A2780 ovarian cancer cells with sfTSLP overexpression (left top); the top 10 gene ontology (GO) enrichment terms of upregulated DEGs (grey bars) and downregulated DEGs (black bars) were shown (left bottom). The upregulated and downregulated DEGs of IGROV-1 and their top 10 GO enrichment of ovarian cancer cells with sfTSLP overexpression (middle top and bottom). The upregulated and downregulated DEGs and their top 10 GO enrichment of HEC1A endometrial cancer cells with sfTSLP overexpression (right top and bottom). (b) The Venn diagram shows relations of DEGs expression by sfTSLP overexpression in A2780 cells (ATvsAM: A2780 cells with stable transfection of pZERO-sfTSLP vs. A2780 cells with stable transfection of pZERO-mcs), IGROV-1 cells (ITvsIM: IGROV-1 cells with stable transfection of pZERO-sfTSLP vs. IGROV-1 cells with stable transfection of pZERO-mcs) and HEC1A cells (HTvsHM: HEC1A cells with stable transfection of pZERO-sfTSLP vs. HEC1A cells with stable transfection of pZERO-mcs). (c) Heatmap of the mRNA expression levels of 11 DEGs in three cancer cell lines (A2780, IGROV-1 and HEC1A) with sfTSLP overexpression. (d) mRNA expression of TSLPv2, EFNB2 and PBX in ovarian (A2780 and IGROV-1) and endometrial (HEC1A) cancer cells with stable transfection of pZERO-sfTSLP or pZERO-mcs was examined by TaqMan expression assays. Data shown (d) are expressed as mean ± S.E.M. from three independent replicates (* p < 0.1, ** p < 0.01, *** p < 0.001, **** p < 0.0001).