Skip to main content
. 2021 Feb 24;22(5):2242. doi: 10.3390/ijms22052242

Figure 2.

Figure 2

Chondrocyte migration and attachment to COMP and TSP-4. (a) Recombinant (rec) COMP and TSP-4 were eluted from the columns in several steps and every protein fraction (F) analyzed by immunoblot. (b) Schematic illustration of the transwell system. Porcine chondrocytes were added to the upper compartment and allowed to migrate towards COMP (10 µg/mL) or TSP-4 (10 µg/mL) for 10 h. (c) Representative images of migrated chondrocytes stained with DAPI. (d) Migrated cells were counted and cell numbers statistically evaluated. Each bar shows the mean + SD and significance (to medium: p < 0.01 ** and to PDGF-BB: p < 0.05 $) was analyzed. (e) Representative images of attached chondrocytes stained with crystal-violet. (f) Attached cells were counted and cell numbers statistically evaluated. Each bar shows the mean + SD and significance (to BSA p < 0.05 *, p < 0.01 **, to fibronectin p < 0.05 $ and COMP p < 0.01 ~~) was analyzed. The standard medium was used as a negative and PDGF-BB (10 ng/mL) as a positive control for the migration assay. BSA (100 mg/mL) was used as a negative control and fibronectin (10 µg/mL) as a positive control for cell attachment. (n = 4–5); scale bar = 100 µm.