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Erk and Smad phosphorylation in chondrocytes after stimulation with COMP and TSP-4. Chondrocytes were stimulated with 10 µg/mL COMP or TSP-4. Cell extracts were harvested and Smad as well as Erk proteins detected by immunoblot. (a) Representative immunoblots showing the detection of pErk1/2 and total Erk1/2. (b) Representative immunoblots showing the detection of pSmad1/5/9, Smad1/5/9, pSmad2 and Smad2. GAPDH was used as a loading control; (n = 4).
