Figure A1.
Surface plasmon resonance measurements show a direct interaction of COMP and TSP-4 with TGF-β1. Protein-protein interaction profiles showing the binding of COMP and TSP-4 to TGF-β1. Surface plasmon resonance sensorgrams generated with the Biacore system are shown for COMP (a) and TSP-4 (b). Resonance signals expressed as response units (RU), indicate the degree of binding COMP and TSP-4 with TGF-β1. COMP and TSP-4 were used in concentrations ranging from 5 to 160 nM and injected to immobilized TGF-β1 on a CM5 chip. (c) Summary of the data received from Biacore assays. The association rate (ka), the dissociation rate (kd), the dissociation constant (Kd) and the chi2 are represented. A kinetic 1:1 model (Langmuir model) was used to calculate these parameters.