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. 2021 Feb 24;22(5):2242. doi: 10.3390/ijms22052242

Figure A2.

Figure A2

The effect of TGF-β1 on COMP and TSP-4 synthesis and deposition. Chondrocytes were stimulated with TGF-β1 (0.5 ng/mL) for seven days. COMP and TSP-4 were detected with specific antibodies. (a) Immunofluorescence staining of COMP and TSP-4 at day 10. (b) Immunoblot assays of chondrocyte supernatants at day 10. Roti®blue staining of proteins was used as a loading control (LC). (n = 3 per group); unstimulated cells were used as a control; scale bar = 100 µm.