Table 1.
List of primary antibodies.
| Target | Host | Dilution | Source or Supplier |
|---|---|---|---|
| Collagen I | rabbit | 1:200 (IF), 1:1000 (IB) | Abcam (ab34710) |
| Collagen II | mouse | 1:200 (IF), 1:1000 (IB) | Merck (CP18) |
| Collagen IX | guinea pig | 1:200 (IF), 1:1000 (IB) | [77] |
| Collagen X | mouse | 1:50 (IF), 1:100 (IB) | Mengjie Zhou (University of Cologne) |
| Collagen XII | rabbit | 1:200 (IF), 1:1000 (IB) | Manuel Koch (University of Cologne) |
| COMP | rabbit | 1:100 (IF), 1:500 (IHC), 1:1000 (IB) | [78] |
| TSP-4 | rabbit | 1:100 (IF), 1:500 (IHC) | [39] |
| TSP-4 | guinea pig | 1:1000 (IB) | [40,79] |
| Smad2 | rabbit | 1:1000 (IB) | Cell Signaling (5339) |
| pSmad2 | rabbit | 1:2000 (IB) | Cell Signaling (3108) |
| Smad1/5/9 | rabbit | 1:500 (IB) | Santa Cruz (sc-6031-R) |
| pSmad1/5/9 | rabbit | 1:1000 (IB) | Cell Signaling (13820) |
| Erk1/2 | mouse | 1:2500 (IB) | Cell Signaling (9107) |
| pErk1/2 | rabbit | 1:2000 (IB) | Cell Signaling (4370) |
| GAPDH | mouse | 1:2000 (IB) | Thermo Fisher Scientific (MA5-15738) |