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. 2021 Feb 25;22(5):2268. doi: 10.3390/ijms22052268

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Multiple Sequence Alignment Analysis of RBD Mutants. (A) Only the target mutation sites are shown, and the non-target amino acids (AA) has been cut out from the alignment representation. The alignment coloring scheme is based on the chemical properties of each AA (each SARS-CoV-2 AA is green, the AA from the same chemistry is orange, and AA with different chemistry is red. WebLogo (http://weblogo.threeplusone.com (accessed on 1 February 2021)) representation shows a relative scale of the presence of each AA in each column. (B) Cross species comparison of RBD amino acids in SARS-CoV-2, Pangolin-CoV 2020, and Bat-CoV-RaTG13. Selected mutants are identified by colored boxes. Colored changes indicate RBD AA differences (V445A and F486A and Y505A).