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. 2021 Feb 27;22(5):2383. doi: 10.3390/ijms22052383

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Determination of dead cell populations after exposure to MCTG107b (a,c) and MCTG214(3b1) (b,d) derived biosurfactants in the HaCaT and THLE3 in vitro skin and liver models, respectively. Both cell lines were incubated with increasing concentrations (0.25–3 mg/mL) of (a,c) MCTG107b and (b,d) MCTG214(3b1) for 24, 48, or 72 h. Levels of dead cells were determined by utilizing the propidium iodide assay. The results are shown as the mean ± SD and are representative from three independent experiments. Note: ** p < 0.01, *** p < 0.001 vs. control (untreated cells).

Determination of dead cell populations after exposure to MCTG107b (a,c) and MCTG214(3b1) (b,d) derived biosurfactants in the HaCaT and THLE3 in vitro skin and liver models, respectively. Both cell lines were incubated with increasing concentrations (0.25–3 mg/mL) of (a,c) MCTG107b and (b,d) MCTG214(3b1) for 24, 48, or 72 h. Levels of dead cells were determined by utilizing the propidium iodide assay. The results are shown as the mean ± SD and are representative from three independent experiments. Note: ** p < 0.01, *** p < 0.001 vs. control (untreated cells).