Figure 5.

Cytokine releases and inflammatory status in corrected FD and FD-ECs. (A) Cytokine levels in conditioned media of corrected FD and FD-ECs were measured using a human cytokine Multi-Analyst ELISArray Kit and (B) normalized to the conditioned media of isogenic control. (C) Expression levels of pro-inflammatory genes validated by qRT-PCR (n = 3 images from 3 biological replicates). (D) Representative images for monocyte (THP-1) adhesion in corrected FD and FD iPSC-ECs. HUVECs were used as a control (ctrl). (E) Quantification of THP-1 adhesion of experiments (n = 3 images from 3 biological replicates). * Compared to corrected FD individually. ^# Compared to PBS in TNFα treatment. The error bars represent SD. One-way ANOVA was used for statistical analysis (* p < 0.05; ** p < 0.01; ^# p < 0.05).