Figure 3.

PGDM-exposed males, but not females, use more oxygen for ATP production, and while mitochondrial transfer may benefit males, it may put females at greater risk of ROS production. (A) Basal, FCCP-stimulated maximal, and reserve respiratory capacities and (B) respiratory control ratios, oxygen consumed in ATP production, and proton leak of male cardiomyocytes (CM) determined by mitochondrial stress test supplemented with mitochondrial transfer. (C,D) Respiratory parameters from PGDM-exposed CM of both sexes. N = 4–6 per group. Data represent mean ± SEM. P ≤ 0.05: ⁺ diabetes effect by 2-way ANOVA, ^# mitochondrial effect by 1-way ANOVA. ^## P < 0.01, ^### P < 0.001.