Skip to main content
. 2021 Feb 27;22(5):2382. doi: 10.3390/ijms22052382

Figure 6.

Figure 6

PGDM-exposed cardiomyocytes of both sexes are at greater risk of stress-induced apoptosis; mitochondrial transfer lowers this risk in males but worsens it in females. (A) Representative CM labeled with 4′,6-diamidino-2-phenylindole (DAPI) and transferase dUTP nick end labeling (TUNEL) for apoptotic cell death following FCCP Challenge. DNase treatment was used as a positive control. TUNEL reaction without enzyme was used as a negative control. (B) Percentage of TUNEL-positive cells following FCCP Challenge. Males have higher numbers of TUNEL-positive cells regardless of group (P = 0.005 by 1-way ANOVA; not demarcated). (C) Difference in TUNEL-positive cells with mitochondrial transfer. N = 4–5 per sex per group using 138 ± 10 cells per plate from 7–10 systematically imaged fields. Data represent mean ± SEM. * P ≤ 0.05 by 1-way ANOVA.