TABLE 2.
Metabarcoding approaches targeting Fusarium communities in environmental samples.
| References | Target gene | Amplicon length (bp) | Fusarium-specific | Sequencing platform | Substrate |
| LeBlanc et al. (2015) | RPB2 | 730 | no | 454 | rhizosphere |
| Walder et al. (2017) | ITS-LSU | 1600 | no | PacBio SMRT | wheat, cover crops and maize |
| Karlsson et al. (2016) | TEF-1α | 550-600 | yes | 454 | wheat kernels, soil |
| Boutigny et al. (2019) | TEF-1α | 640 | yes | Illumina Miseq 2x250 bp | cereal grain |
| Cobo-Díaz et al. (2019) | TEF-1α | 430 | yes | Illumina Miseq 2x300 bp | maize residues, soil |
RPB2, DNA-directed RNA polymerase II second largest subunit; ITS-LSU, internal transcribed spacer region and large subunit of the rRNA; TEF-1α, translation elongation factor 1-alpha.