FIGURE 2.

Conventional fluorescence microscopy images of Cryptococcus neoformans stained with Uvitex2D depicting chitin polymers at the cell wall. (A) Fixed and stained C. neoformans with chitin labeled in blue. Notice the discontinuity of the cell wall staining (red arrow). Those are the specialized regions (SR) in yeasts. (B) Gray scale image of panel (A). Panels (C1–C3) are cells that are under different budding stages (see red arrows). (C) Zoom of panels (C1–C3) (upper panel) with their respective three-dimensional surface plots (lower panel). Surface plots indicate that the cell walls in SRs have a lower fluorescence intensity when compared to the entire cell wall rims. Scale bars are all 10 μm.