Skip to main content
. 2021 Mar 1;12:609244. doi: 10.3389/fmicb.2021.609244

FIGURE 3.

FIGURE 3

Structured Illumination Microscopy images of Cryptococcus neoformans confirm that both, the cell wall and capsule, deform prior to the budding event and generation of daughter cells. Yeast cells were fixed and stained for Uvitex2B (blue) and 18B7 + Alexa Fluor® 594 antibody (red) so we could follow their walls and PS capsules, respectively, during budding and generation of daughter cells. (A) 2.5D yeast profile, showing that the mother cell (left) has a higher cell wall fluorescence intensity when compared to the daughter cell (right). (B) 2D representation of the same field as in panel (A). (C) Representative image of a yeast cell at the beginning of the budding process. SR can be visualized as a discontinuity of the cell wall. (D) Three-dimensional profile of a representative yeast cell also in the beginning of the budding process showing the SR (highlighted in green). Scale bars: panel (A) 1 μm; panels (B–D) 2 μm (Supplementary Video 2).