Figure 11.

ETV2 is involved in the migration, invasion, and epithelial-mesenchymal transition (EMT) process of glioma cells. (A) Expression of ETV2 in IHC stained images of glioma tissues compared with adjacent non-tumor tissues was showed. For quantification, three para-cancerous tissues and five glioma sample for tumor groups were counted, mean values with SD are given, p-value is calculated by unpaired t-test, ^**p < 0.01 and ^***p < 0.001. (B) q-PCR showed the relative expression of ETV2 in HEB, SHG44, and A172 cells. (C) Western blot showed the ETV2 siRNA knockdown efficiency in SHG44 and A172 cells. (D) Wound healing assay showed that ETV2 knockdown led to profound impairment in cell migration ability of A172 and SHG44 relative to the control. (E) Invasion assays showed that ETV2 depletion resulted in suppression of A172 and SHG44 cell invasion capability. (F) Vimentin and N-cadherin levels were detected by western blot analysis in A172 and SHG44 cells after ETV2 knockdown with siRNA. For (D,E) mean values with SD form three independent experiments are given. p-value is calculated by unpaired t-test, ^***p < 0.001.