Figure 1.

Paeonol attenuates the inflammation induced by LPS in RAW264.7 cells. A, The structure of paeonol is shown. B, iNOS, TNF‐α and TGF‐β1 levels in RAW264.7 cells treated with LPS, SB or paeonol were tested by using Western blotting. C, iNOS, TNF‐α, TGF‐β1 and IL‐1β levels were tested by using RT‐qPCR. D, IL‐1β and TNF‐α levels in cell culture supernatant were tested by using ELISA. E, Left: the protein level of P65 was tested by using Western blotting. Middle: the mRNA level of P65 was tested by using RT‐qPCR. Right: the promoter activity of P65 was tested by using a luciferase reporter assay. The bar graphs are representative of three independent experiments. *P < .05, ** P < .01 and *** P < .001